Dev127621 3630..3636
نویسندگان
چکیده
The epicardium is essential for mammalian heart development. At present, our understanding of the timing and morphogenetic events leading to the formation of the human epicardium has essentially been extrapolated from model organisms. Here, we studied primary tissue samples to characterise human epicardium development. We reveal that the epicardium begins to envelop the myocardial surface at Carnegie stage (CS) 11 and this process is completed by CS15, earlier than previously inferred from avian studies. Contrary to prevailing dogma, the formed human epicardium is not a simple squamous epithelium and we reveal evidence of more complex structure, including novel spatial differences aligned to the developing chambers. Specifically, the ventricular, but not atrial, epicardium exhibited areas of expanded epithelium, preferential cell alignment and spindle-like morphology. Likewise, we reveal distinct properties ex vivo, such that ventricular cells spontaneously differentiate and lose epicardial identity, whereas atrial-derived cells remained ‘epithelial-like’. These data provide insight into the developing human epicardium that may contribute to our understanding of congenital heart disease and have implications for the development of strategies for endogenous cell-based cardiac repair.
منابع مشابه
Clinical trials in pediatrics
Anne B Chang Author for correspondence: Queensland Children’s Medical Research Institute, Queensland University of Technology, Herston, QLD, Australia and Child Health Division, Menzies School of Health Research, Charles Darwin University, Tiwi, NT, Australia and Queensland Children’s Respiratory Centre, Royal Children’s Hospital, Brisbane, Queensland, Australia Tel.: +61 7 3636 5270 Fax: +61 7...
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